Recombinant SARS-CoV-2 Nucleocapsid 抗体
(1 reference)Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid 抗体 (ABIN6953114)
抗原
See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes and binds the non-linear/conformational epitope of the N protein of SARS CoV and also binds the SARS CoV2 nucleoprotein.
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产品特性
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Recombinant monoclonal antibody to Covid-19 & SARS-CoV Nucleoprotein.
Several anti-SARS-CoV-2 Nucleoprotein antibodies based on clone CR3009 are available:
Human IgG1, IgG2, IgG3, IgA and IgM for use in neutralization assays and as serological controls- ABIN6953060 - Human IgG1
- ABIN6953114 - Human IgG2
- ABIN6953120 - Human IgG3
- ABIN6953115 - Human IgA
- ABIN6953061 - Human IgM
- ABIN6953062 - Rabbit IgG
- ABIN6953112 - Mouse IgG2b
- ABIN6953111 - Mouse IgM
- ABIN6953116 - Human Fab fragment, His tag
- ABIN6953118 - Human F(ab)2 fragment, His tag
- ABIN6953117 - Mouse Fab fragment, His tag
- ABIN6953119 - Mouse F(ab)2 fragment, His tag
- ABIN6953110 - Human IgG1, Fc-Mutant
- AABIN6953113 - Mouse IgG2b, Fc-Mutant
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The harvested supernatents were then purified on protein A columns.
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亚型
- IgG2b
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anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (Whole Molecule) antibody
Animal-Free SARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, IF, FACS 宿主: 小鼠 Monoclonal AB79-E11 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) antibodySARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, IF 宿主: 小鼠 Monoclonal unconjugated
anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) antibodySARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, IF 宿主: 兔 Chimeric CR3018 (03-018) unconjugated Recombinant Antibody
anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) antibodySARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, GICA 宿主: 小鼠 Monoclonal 3C6 unconjugated
anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (AA 1-419) antibodySARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2), Virus ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) (C-Term) antibodySARS-CoV-2 N 适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 山羊 Polyclonal unconjugated
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应用备注
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This antibody is recommended for detection of SARS CoV2 protein N (nucleoprotein). This antibody binds both the nucleocapsid protein of the SARS-CoV and SARS CoV-2 (2019-nCoV). Initial characterization of the antibody for binding to 2019-nCoV was done using ELISA. This antibody shows potential to be used for development of diagnostic assays. Various isotype versions of the antibody namely human IgG1, IgG3, IgM, IgA and the less common IgG2 and IgG4 are available for the investigation of their role in response to SARS CoV2. Competitive ELISA of this antibody with CR3009 suggests that both these antibodies bind different epitopes of the N protein of SARS CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Immunofluorescence staining was used to demonstrate binding of CR3018 to SARS-CoV infected Vero cells.
Competitive ELISA of both anti-nucleocapsid antibodies suggests that they bind different, non-overlapping epitopes of the N protein of SARS-CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Clone CR3018 binds the amino acid residues between 11-19 of the N protein of SARS-CoV, while clone CR3009 binds a non-linear/conformational epitope of the N protein of SARS-CoV, both of which are sufficiently conserved to permit binding of these antibodies to SARS-CoV-2. -
说明
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This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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: "Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus." in: Journal of virology, Vol. 79, Issue 3, pp. 1635-44, (2005) (PubMed).
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: "Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus." in: Journal of virology, Vol. 79, Issue 3, pp. 1635-44, (2005) (PubMed).
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- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
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别名
- SARS-CoV-SARS-CoV-2 Nucleocapsid Protein2 N
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物质类
- Viral Protein
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背景
- NP, NC, Protein N, Nucleocapsid protein, SARS-CoV Protein N, SARS-CoV Nucleocapsid protein, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV
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UniProt
- P0DTC9
抗原
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